Key features and details | |
Cat. No. | MABL-722 |
Name | Anti-CD147 mAbs |
Clone No. | AFD- 5F6 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Blocking, crystallization, in vivo, inhibition, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | The antibody binds the N domain of CD147. The antibody binds to an epitope on the extracellular domain of CD147 (residues Asp65-Phe74 and Val30-Thr40). CD147 is a type I membrane glycoprotein and is classified into the immunoglobulin-like superfamily, and is expressed in many types of cancer. It is suggested that CD147 is also associated with cancer metastasis, invasion, and chemical drug resistance. |
Alternative Name | Basigin; Emmprin; 5F7; Collagenase stimulatory factor; Extracellular matrix metalloproteinase inducer; Hepatoma-associated antigen; HAb18G; Leukocyte activation antigen M6; OK blood group antigen; Tumor cell-derived collagenase stimulatory factor; TCSF;BSG; Cluster of differentiation 147 |
UniProt | P35613 |
Immunogen | The original antibody was generated by immunizing mice with CD147. |
Application Notes | The specificity of the antibody was coonfirmed by competition ELISA. The antibody recognized MDA-MB-231 cells that express native CD147 antigens on tumor cell surface. To assess the ability of the original antibody to block MMP-1 production, NHLF were treated with recombinant a ECD-Flag construct and the MMP-1 production in response to CD147 stimulation was determined using the MMP-1 activity assay. The antibody showed significant inhibition of MMP-1 production. The original antibody was screened for the ability to inhibit CD147-induced MMP-2 or MMP-9 production in co-culture. Results showed the antibody inhibited MMP-2 and MMP-9 production in co-culture. The humanized version of the antibody (IgG1) inhibited CD147-stimulated MMP-1 production by fibroblast in a dose-dependent manner and the MMP-2 production by MDA-MB-231 and NHDF in a dose dependent manner. |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
