Key features and details | |
Cat. No. | MABL-748 |
Name | Anti-CD172b / SIRPB mAbs |
Clone No. | AFD- OX-130 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | SPR, ELISA, FC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody recognizes both common alleles of the human activating SIRPβ1 receptor, does not recognize SIRPγ, but cross-reacts with one of the common alleles of the inhibitory human SIRPα receptor. |
Alternative Name | SIRP-beta-1; Signal-regulatory protein beta-1; CD172 antigen-like family member B |
UniProt | O00241 |
Immunogen | Recombinant SIRPβ1 was purified by nickel affinity chromatography and used to immunize mice. |
Application Notes | This antibody binds both common alleles of the human activating SIRPβ1 receptor, but ELISA and surface plasmon resonance results have shown that it reacts with one of the two common alleles of SIRPα, potentially yielding different outcomes when this antibody is used in assays from different individuals. |
Antibody First Published | Hatherley et al. OX130 Monoclonal Antibody Recognizes Human SIRPβ1 but Cross-Reacts on SIRPα from One Allele Monoclon Antib Immunodiagn Immunother. 2016 Feb 1; 35(1)： 57–59. PMID：26871367 |
Note on publication | Describes the original production of monoclonal antibody OX130, and the characterisation of its specificity using ELISA and SPR. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
