Key features and details | |
Cat. No. | MABL-747 |
Name | Anti-CD172A/SIRPA mAbs |
Clone No. | AFD- OX-41 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional, IP, WB, ELISA, IF, IHC |
Species Reactivity | Rat |
Basic Information | |
Specificity | This antibody binds to rat SIRP alpha, which is selectively expressed by myeloid and neuronal cells. |
Alternative Name | SIRPA; BIT; MFR; MYD-1; MYD1; P84; PTPNS1; SHPS1; SIRP; Signal- regulatory protein alpha; signal regulatory protein alpha; OX41; MRC OX-41; MRC OX 41 |
UniProt | P97710 |
Immunogen | Rat resident peritoneal cells injected into BALB/c mice. |
Application Notes | This antibody binds to SIRP alpha, and can consequently be used to label neuronal cells and myeloid cells, namely macrophages, monocytes, granulocutes and dendritic cells, in immunofluorescence and immunohistochemical studies (Robinson, 1986). Anti-SIRP ligation induces nitric oxide synthesis and oxidative burst in macrophages (Adams, 1998). |
Antibody First Published | Robinson et al. Macrophage heterogeneity in the rat as delineated by two monoclonal antibodies MRC OX-41 and MRC OX-42, the latter recognizing complement receptor type 3.Immunology 1986 57 239-247 PMID：3512425 |
Note on publication | Describes the original use of this antibody to detect SIRP alpha in labelling, immunoperoxidase and immunofluorescence studies to investigate macrophage heterogeneity. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
