Anti-CD171 mAbs

Cat. No.

MABL-746

Name

Anti-CD171 mAbs

Clone No.

AFD- L1-14.10

From

Recombinant Antibody

Isotype

Engineer antibody

Application

 IP, WB, FC, IF, IHC

Species Reactivity

Human

Basic Information

Specificity

This antibody is specific for the ectodomain   (amino acids M1 to E1123) of human L1, and recognises an epitope in the third   Ig domain. L1 is over-expressed in a range of tumours, including   neuroblastomas, renal carcinomas, ovarian and endometrial carcinomas, and   melanomas. This antibody does not cross-react with human CHL1.

Alternative Name

L1CAM; 14.10; L1; Neural cell adhesion   molecule L1; N-CAM-L1; NCAM-L1

UniProt

P32004

Immunogen

This antibody was raised by immunising mice   with recombinant L1-Fc fusion protein, consisting of the ectodomain of human   L1.

Application Notes

The specificity of this antibody has been   confirmed in flow cytometric (Schafer et al, 2012; Huszar et al, 2006) and   Western blot analysis (Huszar et al, 2006; Wolterink et al, 2010). This   antibody has been shown, when incubated with SKOV3ip human ovarian carcinoma   cells, to efficiently and sustainedly block extracellular signal-regulated   kinase (Erk) phosphorylation (Gast et al, 2008). This antibody has been used   in immunohistochemical staining for L1 expression, using paraffin-embedded   human ovarian carcinoma tissue sections (Wolterink et al, 2010; Knogler et   al, 2007) and a panel of human tumour tissue sections, including benign and   malignant tumours of the female genital tract (Huszar et al, 2006), and renal   cell carcinoma, schwannoma, gastric conventional GIST, pancreatic   neuroendocrine tumor and paraganglioma (Inaguma et al, 2016). This antibody,   when administered to SCID mice in combination with cytostatic drugs,   efficiently reduces the growth of subcutaneously grown Colo357 or SKOV3ip   tumors (Schafer et al, 2012).

Antibody First Published

Huszar et al. Expression profile analysis in   multiple human tumors identifies L1 (CD171) as a molecular marker for   differential diagnosis and targeted therapy. Hum Pathol. 2006 Aug;37(8)：1000-8. Epub 2006 Jun   21. PMID：16867862

Note on publication

Describes the original generation of this   antibody, and its use in immunohistochemical analysis.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.