Key features and details | |
Cat. No. | MABL-750 |
Name | Anti-CD18 mAbs |
Clone No. | AFD-60.3 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | inhibit, WB, Block, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds the CD18 component of the β-2 integrin family which is expressed by leukocytes and neutrophils. |
Alternative Name | ITGB2; MFI7; Integrin beta-2; Cell surface adhesion glycoproteins LFA- 1/CR3/p150.95 subunit beta; Complement receptor C3 subunit beta; β2-subunit of leukocyte integrins |
UniProt | P05107 |
Immunogen | The original antibody was generated by immunizing BALB/c mice with alloantigen activated T cells. |
Application Notes | This antibody is capable of inhibiting several cell mediated immune functions. It inhibits proliferative responses of T cells stimulated by soluble antigens, mitogens, or allogeneic cells in the initiation of a culture, but not when added after 48 hours. It successfully blocks the lytic activity of cytotoxic T cells and natural killer cells and the migration of neutrophilic granulocytes (PMID: 6644024). This antibody inhibits phagocytosis by reaction with the C3bi receptor, and stimulates phagocytosis by reverse opsonization (PMID: 3880787). This antibody inhibits aggregation of neutrophils in response phorbol myristate acetate, zymosan-activated plasma, and N-formyl-methionylleucylphenylalanine in a dose dependent manner (PMID: 3995185). 60.3 also inhibits polymorphonuclear leukocyte accumulation and plasma leakage in vivo (PMID: 3539230). 60.3 successfully inhibits neutrophil function in vivo and has the potential to be used as an anti-inflammatory agent (PMID: 3693901). |
Antibody First Published | Beatty et al. Definition of a Common Leukocyte Cell-Surface Antigen (Lp95- 150) Associated With Diverse Cell-Mediated Immune Functions. J Immunol. (1983); 131(6):2913-8.PMID:6644024 |
Note on publication | Describes the generation and epitope mapping of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
