Key features and details | |
Cat. No. | MABL-751 |
Name | Anti-CD18 mAbs |
Clone No. | AFD- H52 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | inhibit, IP, Block, FC |
Species Reactivity | Dog, Pig, Human |
Basic Information | |
Specificity | This antibody binds disulfide-bonded loop (C386and C400) in the beta-2 integrin subunit. It is reported that the epitope of H52 is masked in the integrin Mac-1 and p150,95. |
Alternative Name | ITGB2; MFI7; Integrin beta-2; Cell surface adhesion glycoproteins LFA- 1/CR3/p150.95 subunit beta; Complement receptor C3 subunit beta; β2-subunit of leukocyte integrins |
UniProt | P05107 |
Immunogen | The original antibody was generated by immunizing BALB/c mice with human splenic adherent cells and later boosted with the same type of cells. |
Application Notes | This antibody is successfully capable of inhibiting T cell, natural killer cells and complement receptor type 3 functions. Binding specificity of this antibody was confirmed with immunoprecipitation (PMID: 2580022). H52 mAb blocked 8E5-PHA-blast fusion in a dose-dependent manner with complete inhibition observed at concentrations above 3 μg/ml. Cell fusion (syncytium formation) is a major cytopathic effect of infection by human immunodeficiency virus (HIV). To determine the effect of the mAb on syncytium formation the LFA-1 expression of 8E5 cells and PHA-blasts was checked by flow cytometry. This mAb inhibited HIV-induced syncytium formation, however it did not block binding of gp120 to CD4 (PMID: 2543075). This mAb cross reacts with procine LFA-1 and profoundly inhibited mitogenic and allogenic stimulation of porcine T lymphocytes as well as cytotoxic T cell and natural killer (NK) cell function (PMID: 2481234). |
Antibody First Published | Hildreth et al. The Human Lymphocyte Function-Associated (HLFA) Antigen and a Related Macrophage Differentiation Antigen (HMac-1): Functional Effects of Subunit-Specific Monoclonal Antibodies J Immunol (1985);134(5):3272-80. PMID:2580022 |
Note on publication | Describes the structural and functional relationship between alpha and beta subunits of HLFA and HMac-1 with use of 5 antibodies that react with these proteins. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
