Key features and details | |
Cat. No. | MABL-762 |
Name | Anti-CD19 mAbs |
Clone No. | AFD- FMC63 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Depletion, FACS, in vivo, IP, FC, IF, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for human CD19, a 95 kDa protein expressed in the B-lymphocyte lineage. |
Alternative Name | B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; Differentiation antigen CD19; Leu12; T-cell surface antigen Leu-12 |
UniProt | P15391 |
Immunogen | Human prolymphocytic leukaemia cell line JVM3. |
Application Notes | FMC63 was used for four years as a CD19 antibody in various labs prior to its original publication. There, it was reported that the antibody percipitates CD19 and inhibits the binding of other CD19 antibodies to it (Zola et al., 1991; PMID: 1725979). FMC63 was used for IF and FACS of peripheral blood mononuclear cells (PBMC) from patients with multiple myeloma (MM) (Szczepek et al., 1997; PMID: 9057669) and receptor for hyaluronan (HA)-mediated motility (RHAMM) expressing malignant B-lineage cells in myeloma (Crainie et al., 1999; PMID: 10029598). FMC63 was used for IHC and IIP of B cells in lymphocyte-filled villi and IIP of jejunal isolated lymphoid follicle, respectively, in small bowel specimens from human patients (Moghaddami et al.,1998; PMID: 9834269). FNC63 was used to induce B cell depletion in vivo (DiLillo et al., 2011; PMID: 21248259). |
Antibody First Published | Zola et al. Preparation and characterization of a chimeric CD19 monoclonal antibody Immunol Cell Biol. 1991 Dec;69 ( Pt 6):411-22. doi: 10.1038/icb.1991.58 PMID:1725979 |
Note on publication | The original publication discusses the production of FMC63, a mouse-human chimera of the CD19 cluster monoclonal antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
