Anti-CD20 mAbs

Cat. No.

MABL-779

Name

Anti-CD20 mAbs

Clone No.

AFD- 1F5

From

Recombinant Antibody

Isotype

Engineer antibody

Application

depleting, stimulatory, therapeutic

Species Reactivity

Human

Basic Information

Specificity

This antibody is specific for CD20, which is expressed by normal and malignant B cells.

Alternative Name

MS4A1; B-lymphocyte antigen CD20;   B-lymphocyte surface antigen B1; Bp35; Leukocyte surface antigen Leu-16;   Membrane-spanning 4-domains subfamily A member 1; E9PPL6; P19437

UniProt

P11836

Immunogen

This antibody was raised by hyperimmunising BALB/c mice with the Raji Burkett's lymphoma line and the NALM-6 pre B leukaemia line.

Application Notes

This antibody, either alone or in   combination with T cell derived factors, induces B cell proliferation via   CD20 cross-linking (Clark et al, 1985).When administered to patients with  refractory malignant B cell lymphomas, this antibody depletes circulating and   lymph node tumour cells, with minimal toxicity (Press et al, 1987). A   murine/human chimeric IgG1 construct of this antibody has been shown to lyse   Burkitt's lymphoma (5RAJI) cells (Stockmeyer et al, 2000), while IgG1 and  IgA2 isotypes of this antibody have been shown to kill B cells in vitro and   in deplete B cells in vivo (Lohse et al, 2018). The humanized version of this   antibody can be provided upon request.

Antibody First Published

Ledbetter et al. Surface phenotype and   function of tonsillar germinal center and mantle zone B cell subsets Human   Immunology, 1986, Vol.15(1), pp.30-43 PMID：2419284

Note on publication

Describes the original generation of this   antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.