Key features and details | |
Cat. No. | MABL-780 |
Name | Anti-CD20 mAbs |
Clone No. | AFD- 2H7 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, FC, IF |
Species Reactivity | Baboon, chimpanzee, gibbon, langur, Marmoset, owl monkey, Human |
Basic Information | |
Specificity | This antibody is specific for CD20, which is expressed by normal and malignant B cells. |
Alternative Name | MS4A1; B-lymphocyte antigen CD20; B-lymphocyte surface antigen B1; Bp35; Leukocyte surface antigen Leu-16; Membrane-spanning 4-domains subfamily A member 1; E9PPL6; P19437 |
UniProt | P11836 |
Immunogen | This antibody was raised by hyperimmunising a C57BL/6 mouse with the Ia- B cell line 6.16 cl. 3. |
Application Notes | This antibody has been used in flow cytometry and quantitative immunofluorescence analysis of human peripheral blood mononuclear leukocytes (Yanase et al, 1986).It has also been used to precipitate CD20 from lysates of the Raji human B cell line (Deans et al, 1995). This antibody has been exploited as part of a an avidin-biotin immunoadsorption system to eliminate Daudi lymphoblasts from human bone marrow (Berenson et al, 1986). A mouse-human chimeric version of this antibody effectively mediates antibody-dependent cellular cytotoxicity with human effector cells and complement-dependent cytotoxicity with human complement (Liu et al, 1987). |
Antibody First Published | Ledbetter et al. Surface phenotype and function of tonsillar germinal center and mantle zone B cell subsets Human Immunology, 1986, Vol.15(1), pp.30-43 PMID：2419284 |
Note on publication | Describes the original generation of this antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
