Anti-CD20 mAbs

Cat. No.

MABL-782

Name

Anti-CD20 mAbs

Clone No.

AFD- ABM58020-32A8-PU

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB, ELISA, IHC

Species Reactivity

Human

Basic Information

Specificity

This antibody binds human CD20. CD20 is a   33kDa tetraspanning transmembrane phosphoprotein which is expressed in pre   B-cells. This B-lymphocyte-specific membrane protein plays a role in the   regulation of cellular calcium influx necessary for the development,   differentiation, and activation of B-lymphocytes. It also functions as a   store-operated calcium (SOC) channel component promoting calcium influx after   activation by the B-cell receptor/BCR.

Alternative Name

Bp35; Leu-16; MS4A1; Leukocyte surface   antigen Leu-16; B-lymphocyte antigen CD20; B-lymphocyte surface antigen B1;   Membrane-spanning 4-domains subfamily A member 1; L26

UniProt

P11836

Immunogen

The original antibody was generated by immunizing   BALB/c mice with CD20-KLH conjugate in Freund's complete adjuvant.

Application Notes

This binding specificity of this antibody   for human CD20 was checked using ELISA. This antibody positively binds human   CD20-BSA in an ELISA. The affinity constant of the original IgG2b antibody   was determined to be 3.07 × 10^10 M^(-1). This antibody can recognize human   CD20 in an immunoblot assay. This antibody is similar to commercially   available anti-CD20 antibody L26 in immunohistochemical staining of B cell  lymphomas. This antibody was used in the immunohistochemical staining of B   cell lymphomas, T cell lymphomas, tonsil tissue and appendectonic tissues (CN111363043).

Antibody First Published

Note on publication

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.