Key features and details | |
Cat. No. | MABL-783 |
Name | Anti-CD20 mAbs |
Clone No. | AFD- B9E9 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | radioimmunotherapy, WB |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for CD20, which is expressed by normal and malignant B cells. It does not cross-react with murine CD20. |
Alternative Name | MS4A1; B-lymphocyte antigen CD20;MS4A1; B-lymphocyte surface antigen B1; Bp35; Leukocyte surface antigen Leu-16; Membrane-spanning 4-domains subfamily A member 1; E9PPL6; P19437 |
UniProt | P11836 |
Immunogen | This antibody was raised against human CD20. |
Application Notes | This antibody has been used in western blot detection of CD20 in human plasma, revealing elevated CD20 levels in plasma from chronic lymphocytic leukemia patients (Manshouri et al, 2003). An scFv derived from this antibody fused to streptavidin has been investigated as a method of pretargeted radioimmunotherapy for lymphomas (Schultz et al, 2000), and has been demonstrated to be non-toxic in a phase I trial of non-Hodgkin lymphoma patients (Forero et al, 2004). Additionally, measles viruses engineered to express this scFv are able to enter CD20+ target cells through interaction with CD20 antigen, with clinical relevance for lymphoma therapy (Bucheit et al, 2003). |
Antibody First Published | Schultz et al. A Tetravalent Single-chain Antibody-Streptavidin Fusion Protein for Pretargeted Lymphoma Therapy Cancer Res. 60 (23), 6663-6669 (2000) PMID：11118050 |
Note on publication | Describes the generation of an scFv from this antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
