Key features and details | |
Cat. No. | MABL-824 |
Name | Anti-CD24 mAbs |
Clone No. | AFD- G7 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, ELISA, FC, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds human CD24. |
Alternative Name | Signal transducer CD24; Small cell lung carcinoma cluster 4 antigen |
UniProt | P25063 |
Immunogen | The original antibody was generated by immunizing BALB/c mice with a CD24 core region polypeptide and hemocyanin KLH conjugate (CD24-KLH) as immunogen. |
Application Notes | This antibody showed specific binding affinity to recombinant human CD24 but not to unrelated recombinant Fc in a western blot. The binding specifity of G7 towards CD24 was also confirmed with competitive ELISA. Flow cytometric assay was carried out to assess the affinities of G7 to native CD24 expressed on Huh7 and HT29 cells with HCT116 cells as control. G7mAb demonstrated significant binding to both Huh7 and HT29 cells with rates 52.9% and 41.3% respectively. This antibody was also used in immunohistochemical staining of tumor samples from Huh7 xenograft nude mice (PMID: 26255089). |
Antibody First Published | He et al. A novel antibody targeting CD24 and hepatocellular carcinoma in vivo by near-infrared fluorescence imaging. Immunobiology. (2015); 220(12):1328-36. PMID:26255089 |
Note on publication | Describes the generation of an scFv version of this antibody and tests its use in targeted tumor therapy and diagnostic potentials in vitro and in vivo. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
