Key features and details | |
Cat. No. | MABL-835 |
Name | Anti-CD257 (BAFF) mAbs |
Clone No. | AFD- 5B7 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Block, FC |
Species Reactivity | Human, Cynomolgus Monkey, Mouse |
Basic Information | |
Specificity | This antibody binds human BAFF protein and cross reacts with mouse and cynomolgus monkey BAFF protein. B-cell-activating factor (BAFF/CD257) is a cytokine belonging to the tumour-necrosis factor (TNF) family. This cytokine binds to TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R. BAFF is a survival factor for B cells that controls B-cell maturation. It is also involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. |
Alternative Name | BLyS; TNFSF13B; TALL-1; TNFSF20; ZTNF4; Tumor necrosis factor ligand superfamily member 13B; B lymphocyte stimulator; B-cell-activating factor; Dendritic cell-derived TNF-like molecule; TNF and APOL-related leukocyte expressed ligand 1 |
UniProt | Q9Y275 |
Immunogen | The original antibody was isolated from a naïve human scFv phage library using multiple panning strategies including solid-phase panning with immobilized CD47 and solution-phase panning using biotinylated CD47. |
Application Notes | This antibody is capable of reacting with both soluble and membrane bound human, mouse and cynomolgus monkey BAFF/ BLyS protein. The binding ability of this antibody to membrane bound CHO-human BLys, HEK293-mouse BLyS and CHO-cynomolgus monkey BLyS protein was determined using flow cytometry. This antibody is capable of blocking the interaction between human BAFF and BR3.This antibody was also capable of neutralizing BLyS-mediated B-cell proliferation. The binding affinity characterization of this antibody was done using SPR. It was reported that the original IgG antibody binds human and mouse BLyS with a binding affinity of Kd= 4X10^(-10) M and Kd= 1.45X10^(-11) M respectively (US20170101466). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
