Anti-CD266 mAbs

Cat. No.

MABL-844

Name

Anti-CD266 mAbs

Clone No.

AFD- P4A8

From

Recombinant Antibody

Isotype

Engineer antibody

Application

inhibition, WB, FC, IHC

Species Reactivity

Human

Basic Information

Specificity

This antibody is specific for human Fn14.

Alternative Name

FN14; TweakR; TNFRSF12A; FGF-inducible 14;   Tweak-receptor; Tumor necrosis factor receptor superfamily member 12A; Fibroblast growth factor-inducible immediate-early response protein 14

UniProt

Q9NP84

Immunogen

The original antibody was raised by   immunizing Fn14-deficient mice with CHO cells expressing human surface Fn14   and boosted with Fn14-myc-His

Application Notes

The original antibody was tested to inhibit   distinct TWEAK-induced cellular effects known to be mediated by Fn14. HT29   and WiDr cells were treated with this antibody, this resulted in a strong   inhibition of TWEAK-induced IL8 production. Furthermore, it was shown that   oligomerization can greatly increase the agonistic activity of this antibody   (Salzmann et al., 2013; PMID：23532848). This antibody was used for flow cytometry on HEK293 cells   transfected with plasmids expressing Fn14 protein. This antibody was used for   a western blot on lysates from HEK293 cells expressing Fn14 (Trebing et al.,   2014; PMID：24135629). This   antibody was used for immunohistochemistry on different human brain tumors   (Tran et al., 2006; PMID：17018610), and on samples from xenografted tumors made from H460 cells   (Jandova et al., 2015; PMID：25563366).

Antibody First Published

Tran et al. Increased fibroblast growth   factor-inducible 14 expression levels promote glioma cell invasion via Rac1   and nuclear factor-kappaB and correlate with poor patient outcome Cancer Res.   2006 Oct 1;66(19)：9535-42. PMID：17018610

Note on publication

The original paper shows that the fibroblast   growth factor-inducible 14 (Fn14), a member of the tumor necrosis factor   (TNF) receptor superfamily, is expressed at high levels in migrating glioma   cells in vitro and invading glioma cells in vivo.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.