Key features and details | |
Cat. No. | MABL-855 |
Name | Anti-CD28 mAbs |
Clone No. | AFD- D665 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | CoStim, SPR, Superagonism, FC, IHC |
Species Reactivity | Mouse |
Basic Information | |
Specificity | This antibody binds to the laterally exposed C′D loop of CD28 and induces T cell proliferation without TCR ligation. |
Alternative Name | TP44; T-cell-specific surface glycoprotein CD28; TP44 |
UniProt | P31041 |
Immunogen | The original D665 antibody was generated by immunizing mice alternately with A20 cells expressing mCD28 i.p. and with recombinant mCD28Ig s.c. |
Application Notes | This antibody binds to mouse CD28. The original mouse IgG1 version was shown to trigger T-cell proliferation in vitro and in vivo in a TCR-independent manner and is a superagonistic antibody. This antibody does not compete with E18 (Ab00286). |
Antibody First Published | Dennehy et al. Cutting Edge: Monovalency of CD28 Maintains the Antigen Dependence of T Cell Costimulatory Responses Journal of Immunology PMID:16670276 |
Note on publication | Cutting Edge: Monovalency of CD28 Maintains the Antigen Dependence of T Cell Costimulatory Responses |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
