Key features and details | |
Cat. No. | MABL-860 |
Name | Anti-CD28 mAbs |
Clone No. | AFD- PV1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, FC |
Species Reactivity | Mouse |
Basic Information | |
Specificity | The antibody binds specifically to murine CD28. |
Alternative Name | T-cell-specific surface glycoprotein CD28; CD28 receptor |
UniProt | P31041 |
Immunogen | The antibody was generated using C57BL/6N mouse T cell lymphoma EL4 cell line as antigen. |
Application Notes | CD28-positive murine T cell line EL4 cells were stained with the antibody and analyzed by flow cytometry (US20040116675A1). Protein lysates from J558 were immunoprecipitated with the antibody (Rozanski et al., 2015; PMID: 25833397). Anti-CD28-PV1-IgG3 is the hamster-mouse chimeric version of the antibody. The antibody enabled long-term survival of heart allografts in rats by reducing the activation of alloantigen-mediated key signaling events in T cells (Jang et al. 2008; PMID: 18408588). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
