Anti-CD28 mAbs

Cat. No.

MABL-859

Name

Anti-CD28 mAbs

Clone No.

AFD- JJ316

From

Recombinant Antibody

Isotype

Engineer antibody

Application

CoStim, Superagonism, FC, IF, IHC

Species Reactivity

Rat

Basic Information

Specificity

 Recognises rat CD28 and can trigger T-cell   proliferation in vitro and in vivo.

Alternative Name

 TP44; T-cell-specific surface glycoprotein   CD28

UniProt

 P31042

Immunogen

Made by immunizing BALB/c mice with rat CD28   cDNA-transfected A20/J cells.

Application Notes

This antibody binds rat CD28 and can convey   as a co-stimulatory signal. At 100-1000- fold higher concentration than   required for co-stimulation JJ316 (unlike the clone JJ319 marketed by   competitors (see Tacke et al. 1997)) can induce proliferation of all T-cell   subsets without TCR engagement.

Antibody First Published

Tacke et al. CD28-mediated induction of   proliferation in resting T cells in vitro and in vivo without engagement of   the T cell receptor: evidence for functionally distinct forms of CD28. Eur J   Immunol PMID:9022025

Note on publication

Describes the in vivo and in vitro   characterisation of JJ316.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.