Key features and details | |
Cat. No. | MABL-873 |
Name | Anti-CD30 mAbs |
Clone No. | AFD- 5F11 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | in vitro, in vivo, SPR, ELISA, FC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for human CD30 |
Alternative Name | TNFRSF8; D1S166E; Ki-1 antigen; CD30L receptor; Lymphocyte activation antigen CD30 |
UniProt | P28908 |
Immunogen | The original antibody was raised by immunzing mice expressing human antibodies with human CD30. |
Application Notes | The original antibody was used for an ELISA on recombinant CD30-Fc fusion protein. This antibody was used for flow cytometry on Hodgkin tumor cell line L540 and on the CD30 line Daudi. This antibody was used for surface plasmon resonance on the antigen sCD30-Fc. The capacity of the antibody to mediate lysis of CD30-expressing tumor cells was investigated using a 51Cr-release assay. It showed the ability to lyse these cells (Borchmann et al., 2003; PMID:12881320). The ability of this antibody to inhibit growth of tumor cells in vitro was demonstrated by a cell viability assay using XTT (Böll et al., 2005; PMID:15878978, Borchmann et al., 2003; PMID:12881320). A clear and dose-dependent effect on cell metabolism could be observed. This antibody was tested in vivo on CD30 expressing human tumors. CD30 expressing human tumors were established in SCID mice then the mice were treated with this antibody.The antibody showed substantial tumor regression and in some cases complete disappearance (Borchmann et al., 2003; PMID:12881320). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
