Anti-CD3 zeta mAbs

Cat. No.

MABL-872

Name

Anti-CD3 zeta mAbs

Clone No.

AFD- G3

From

Recombinant Antibody

Isotype

Engineer antibody

Application

IP, WB, ELISA

Species Reactivity

Human, Mouse

Basic Information

Specificity

This antibody recognises COOH-terminal   region of CD3 zeta which is a part of the TCR-CD3 complex present on all   mature T-lymphocyte cells and is crucial in the adaptive immunity.

Alternative Name

TCR zeta; CD3z; TCRz; CD247; T-cell surface   glycoprotein CD3 zeta chain; T-cell receptor T3 zeta chain; CD3-zeta;   TCR-zeta

UniProt

P20963

Immunogen

This antibody was raised by immunising BALB/c   mice with a 113 amino acid synthetic peptide corresponding to the cytoplasmic   domain of murine CD3 zeta.

Application Notes

G3 antibody might be utilised in the   detection and analysis of T cells as well as in research on lymphoid   malignancies. This antibody was shown to exhibit strong affinity to CD3 zeta   in ELISA and Western blot and to be very effective in precipitating CD3 zeta   (as well as its phosphorylated form) from Jurkat T cell lysate (van Oers et   al., 1994). It is also used

Antibody First Published

van Oers et al. Production and   characterization of monoclonal antibodies specific for the murine T cell   receptor zeta chain. J Immunol Methods. 1994 Apr 15;170(2)：261-8.PMID：7512608

Note on publication

This article describes the generation and   characterisation of G3 antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.