Cat. No.
MABL-1362
Application
FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
9E 8
From
Recombinant Antibody
Specificity
This antibody is specific for human DCIR, which is expressed by monocytes, B cells, neutrophils, granulocytes and dendritic cells.
Alternative Names
CD367; Clec4A; C-type lectin domain family 4 member A; LLIR; CLECSF6; C-type lectin DDB27; C-type lectin superfamily member 6; Dendritic cell immunoreceptor; Lectin-like immunoreceptor
UniProt
Q9UMR7
Immunogen
This antibody was raised by immunising BALB/c mice with a dendritic cell immunoreceptor ectodomain-human Fc fusion protein.
Application Notes
This antibody has been used in flow cytometric analysis of DCIR expression by peripheral blood mononuclear cells (Klechevsky et al, 2010). A recombinant humanised form of this antibody fused to protein antigens is under investigation as an antigen-targeting vaccine (Flamar et al, 2012), and has been shown to trigger antigen-specific CD8+ T cell immunity.
Antibody First Published
Klechevsky et al. Cross-priming CD8+ T cells by targeting antigens to human dendritic cells through DCIR Blood. 2010 Sep 9; 116(10): 1685–1697.
Note on publication
Describes the original generation of this antibody, and preliminary investigation into its use as an antigen-targeting vaccine.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
