Cat. No.
MABL-1365
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Fusarium species
Clone No.
3G2
From
Recombinant Antibody
Specificity
This antibody binds mycotoxin deoxynivalenol (DON). This antibody also shows reactivity with deoxynivalenol analogs 3-AC-deoxynivalenol and 15-AC-deoxynivalenol but not with other trichotecene members such as nivalenol and T2 toxin. Deoxynivalenol belongs to the trichothecene class of mycotoxins, generated by several Fusarium fungi. Deoxynivalenol (DON), along with 3-acetyl-deoxynivalenol (3-ADON) and 15-acetyldeoxynivalenol (15-ADON), occur in grains and cereal products and is often hazardous to humans and livestock. Biochemically, deoxynivalenol is an inhibitor of protein synthesis and has hemolytic activity, causing apoptosis (cytotoxicity) and immunotoxicity to eukaryotic organisms. In animals, deoxynivalenol can cause anorexia and vomiting (PMID: 8896360; 16420629), and it can also cause human skin irritation, hemorrhaging, hematological changes, lymphocyte blastogenesis impairment, radiomimetic effects and apoptosis (PMID: 8896360; 16326049). In plants, deoxynivalenol is a virulence factor in the pathogenesis of G. zeae on wheat (PMID: 16420629).
Alternative Names
DON; Mycotoxin; Vomitoxin, Fusarium mycotoxin; ChEBI: 10022; 1.3G2
UniProt
Immunogen
The original antibody was generated by using phage display technology.
Application Notes
The scFv version of this antibody was conjugated to horseradish peroxidase (HRP) for immunoassay. This HRP conjugated scFv antibody was able to detect deoxynivalenol in spiked and natural contaminated wheat samples in a competitive direct-ELISA (PMID: 17521405).
Antibody First Published
Want et al. Detection of deoxynivalenol based on a single-chain fragment variable of the antideoxynivalenol antibody. FEMS Microbiol Lett. 2007 Jul;272(2):214-9. PMID: 17521405
Note on publication
Describes the development of a immunological detection method for safely diagnosing fungal infection and food contamination.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
