Cat. No.
MABL-1391
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
DD1
From
Recombinant Antibody
Specificity
The antibody recognizes tetrachloro- and pentachloro-dibenzodioxins and -dibenzofurans. It falls to bind either non-chlorinated, mono-, hexa-, or octa-chlorinated dibenzodioxins, nor does it recognize non-chlorinated, octachloro- or 1,2,3,4,8,9-hexachlorodibenzofurans. Dioxins are a group of chemical compounds that are persistent organic pollutants (POPs) in the environment.
Alternative Names
PCDD; DLCs; PCDF; TCDD; DD-1
UniProt
Immunogen
The antibody was generated by immunizing Biozzi mice with 1-N-(adipamino)-2,3,7-triCDD- linked carrier-protein BSA.
Application Notes
The specificity of the antibody for 2,3,7,8-TCDD was confirmed by ELISA analysis. In order to evaluate the ability of the antibody to recognize various kinds of polychlorinated dibenzodioxins and dibenzofurans, a competition ELISA was developed. The antibody recognized 3,3',4,4'-PCB and the single-ring, chlorinated compounds, 2,4,5-trichlorophenol, mixed isomers of hexachlorocyclohexane and 2,5- dinitrobenzene. The antibody is suitable for the development of rapid, inexpensive screening assays for monitoring the presence of dioxin in biological, soil and other environmental samples (Stanker et al., 1987; PMID: 3629609).Further, the recombinant Fab antibody was generated; it displayed competitive inhibition with 2,3,7,8-tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) in an indirect ELISA (Lee et al.; 1998).
Antibody First Published
Stanker et al. Monoclonal antibodies for dioxin: antibody characterization and assay development Toxicology. 1987 Sep;45(3):229-43. PMID:3629609
Note on publication
The paper describes the generation and characterization of a set of 5 anti-dioxin monoclonal antibodies.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
