Cat. No.
MABL-1406
Application
Blocking, ELISA, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
61B
From
Recombinant Antibody
Specificity
This antibody is specific for the EGF-like 3 domain of Dll4.
Alternative Names
Delta-like protein 4; Drosophila Delta homolog 4; Delta4
UniProt
Q9NR61
Immunogen
The original antibody was generated by immunizing mice with soluble human Dll4-His protein.
Application Notes
U87MG and HT29 cells were immune-stained with this antibody (Zhou et al, 2015; pmid:26288060). This antibody is used for immunohistochemistry on U87MG and HT29 (Zhou et al, 2015; pmid: 26288060). The 64Cu-chelator DOTA conjugated version of this antibody was used for the positron- emission tomography (PET) imaging of human glioblastoma and colorectal cancer xenografts (Zhou et al, 2015; pmid:26288060). This antibody was used for immunohistochemistry on spheroids and monolayers cells (US7906116). ELISA was performed with this antibody on Dll4 truncation mutants (US7906116). This antibody is able to block the Dll4-Notch1 interaction (US7906116).
Antibody First Published
Zhou et al. PET Imaging of Dll4 Expression in Glioblastoma and Colorectal Cancer Xenografts Using (64)Cu-Labeled Monoclonal Antibody 61B. Mol Pharm. 2015 Oct 5;12(10):3527-34. PMID: 26288060
Note on publication
A (64)Cu-labeled probe for positron emission tomography (PET) imaging of tumor Dll4 expression was developed.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
