Cat. No.
MABL-1415
Application
EMSA, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
BG4
From
Recombinant Antibody
Specificity
Binds with high selectivity and low nanomolar affinity to DNA and RNA G-quadruplex structures.
Alternative Names
G4 quadruplex
UniProt
n/a
Immunogen
DNA/RNA G-quadruplex.
Application Notes
This antibody has been shown (by ELISA) to bind selectively to G-quadruplex forming DNA and RNA structures. Please that Fab-based versions as well as scFvs, with and without Flag-tag are available, which needs to be considered when replicating published methods. Tsai et al. (2022 et al. PMID: 36440760), report similar activtiy between whole-antibody and scFv-based versions of BG4. Weldon et al. (2016, PMID: 27820800) used Ab00174-1.1 to identify G-quadruplexes in long functional RNAs using an electrophoretic mobility shift assay (EMSA). The mouse IgG1 version of BG4 was shown to have weak cross- reactivity with iMotif structures (2018, PMID: 29686376). iMotif structures can be detected selectively using our anti-iMotif antibody Ab01462.
Antibody First Published
Biffi G, Tannahill D, McCafferty J, Balasubramanian S. Quantitative visualization of DNA G-quadruplex structures in human cells. Nat Chem. 2013 Mar;5(3):182-6. PMID:23422559
Note on publication
Describes generation of a G-quadruplex-specific antibody using phage display and the in vitro selection (using ELISA and CD) of a structure-specific antibody probe that binds with high selectivity and low nanomolar affinity to DNA G-quadruplex structures called BG4.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
