Cat. No.
MABL-1422
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Specie independent
Clone No.
DA24cB7
From
Recombinant Antibody
Specificity
The antibody is specific for domoic acid. Domoic acid is a kainic acid-type neurotoxin that causes amnesic shellfish poisoning. It is produced by algae and accumulates in shellfish, sardines, and anchovies. When sea lions, otters, cetaceans, humans, and other predators eat contaminated animals, poisoning may result.
Alternative Names
DA; (2S,3S,4S)-4-[(2Z,4E,6R)-6-Carboxyhepta-2,4-dien-2-yl]-3- (carboxymethyl)pyrrolidine-2-carboxylic acid
UniProt
Immunogen
The original antibody was originated from a scFv phage library obtained from a sheep hyperimmunised with DA-BSA conjugate. It was isolated by panning against DA.
Application Notes
The specificity of the original format of the antibody was confirmed by competitive ELISA using plates coated with DA-OVA (I50 of 1.21 ng/mL (3.9 nM)). The assay was used for the determination of DA levels in shellfish extracts (Shaw et al., 2008; PMID: 18384202).
Antibody First Published
Shaw et al. Development of a high-affinity anti-domoic acid sheep scFv and its use in detection of the toxin in shellfish Anal Chem. 2008 May 1;80(9):3205-12. doi: 10.1021/ac7024199. Epub 2008 Apr 3. PMID:18384202
Note on publication
The paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
