Cat. No.
MABL-1424
Application
radioimmunotherapy
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
2D12.5
From
Recombinant Antibody
Specificity
This antibody binds metal bound DOTA (Tetraxetan) and also shows binding affinity for Yttrium(III) labeled DOTA, (S) nitro benzyl-DOTA chelates and Janus-DOTA (a bivalent form of DOTA). DOTA is a chelating agent which can be labelled with radioactive yttrium ion and even conjugated with monoclonal antibodies for radioimmunotherapy.
Alternative Names
Tetraxetan; dodecane tetra acetic acid; CHEBI:61028; Yttrium(III) labeled DOTA; Y-DOTA; Yttrium-1 4 7 10-tetraazacyclododecanetetraacetic acid; metal-DOTA
UniProt
Immunogen
The original antibody was generated by immunizing BALB/c mice with KLH-21T-BAD-Y(III).
Application Notes
The original mouse IgG1 antibody binds DOTA chelates of all the rare earths with approximately Kd ≈ 10(-8) M, making it useful for the capture of probe molecules with a variety of properties (PMID: 15546206). The original IgG1 version of this antibody was used in the pretargeting for radioimmunotherapy in BALB/c mice with KHJJ tumors (PMID: 7954426). In vivo clearance studies have demonstrated that 74-96% of 2D12.5 bound to metal chelates is present 24 hours after administration of the bound complex (US7528235). An engineered version of this antibody with a single cysteine residue at position 54, 55 or 56 of the heavy chain, a site proximal to the protein's binding site, enables formation of weakly electrophilic metal complexes of (S)-2-(4-acrylamidobenzyl)-DOTA (AABD) that may bind and form permanent linkages (PMID: 15546206; 15546207). The heavy chain G54C mutant had a small set of electrophilic ligands, differences in reactivity indicated that the substituents near the electrophilic atom can be important determinants of permanent binding (PMID: 15546207).
Antibody First Published
Goodwin et al. Pharmacokinetics of pretargeted monoclonal antibody 2D12.5 and 88Y-Janus-2-(p-nitro benzyl)-1,4,7,10-tetraazacyclododecanetetraacetic acid (DOTA) in BALB/c mice with KHJJ mouse adenocarcinoma: a model for 90Y radioimmunotherapy. Cancer Res. 1994 Nov 15;54(22):5937-46. PMID: 7954426
Note on publication
Describes the pretargeting and pharmacokinetics of antibody 2D12.5 and DOTA for radioimmunotherapy in mouse models.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
