Cat. No.
MABL-1428
Application
in vivo, neutralize, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Bacillus anthracis
Clone No.
F26G3
From
Recombinant Antibody
Specificity
This antibody specifically binds the poly-γ-D-glutamic acid (γDPGA) capsule of Bacillus anthracis. Bacillus anthracis is surrounded by an antiphagocytic polypeptide capsule composed of poly γ-d- glutamic acid (γDPGA). The γDPGA capsule shields the vegetative form of B. anthracis from agglutination by monoclonal antibodies to its cell wall polysaccharide. γDPGA has been identified recently as a potential target for vaccine development. Bacillus anthracis is a spore-forming bacterium and a causative agent for Anthrax, which is a highly lethal infectious disease in human and poses a great threat as an emerging bioterror agent.
Alternative Names
DPGA; capsular antigen; γDPGA; gamma DPGA; poly-gamma-d-glutamic acid; poly-γ-d-glutamic acid; antiphagocytic polypeptide capsule
UniProt
Immunogen
The original antibody was generated by immunizing BALB/c mice with B. licheniformis γDPGA in combination with CD40 agonist antibody.
Application Notes
The binding of this antibody to B. anthracis capsule was assessed by direct immunofluorescence using the Alexa Fluor 488 (Molecular Probes) labeled antibody. An antigen capture ELISA was developed, where this antibody was used as capture antibody and a horseradish peroxidase conjugate of this antibody was used as an indicator antibody. The assessment of assay sensitivity was done using γDPGA from B. licheniformis demonstrated that the assay could detect γDPGA at concentrations as low as 100-140 pg/ml. Passive immunization with this antibody protected >90% of mice in a pulmonary model of anthrax that was lethal in control mice (PMID: 15051894). A 50:50 mixture of PDGA antibodies F24F2 and F26G3 was used in the development of an antigen capture ELISA with a sensitivity of approximately 9 ng/ml of serum after a 1/40 dilution and 2.25 ng/ml for a 1:10 dilution (PMID: 19506008).
Antibody First Published
Kozel et al. MAbs to Bacillus anthracis capsular antigen for immunoprotection in anthrax and detection of antigenemia. Proc Natl Acad Sci U S A. 2004 Apr 6; 101(14): 5042–5047. PMID:15051894
Note on publication
Describes the generation of two antibodies against B. anthracis γDPGA and evaluates their capacity to provide protection in pulmonary mouse model of anthrax.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
