Cat. No.
MABL-1431
Application
in vivo, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
T1014G03
From
Recombinant Antibody
Specificity
This antibody binds human CD261 (TR4 or TRAILR1 or TNFRSF10A). This protein acts as a receptor for the cytotoxic ligand TNFSF10/TRAIL and transduces cell death signal and induces cell apoptosis.
Alternative Names
CD261; TR4; TNFRSF10A; APO2; TRAILR1; Tumor necrosis factor receptor superfamily member 10A; Death receptor 4; TNF-related apoptosis-inducing ligand receptor 1
UniProt
O00220
Immunogen
The original antibody was isolated from a human scFv library by panning against human TR4 fusion protein. Later on the scFv version of this antibody was converted to human IgG1 version for carrying out further studies.
Application Notes
The binding specificity of this antibody to human TR4 (CD126) fusion protein was determined using ELISA. This antibody does not cross react with other proteins like TR7, TR5, TR10 and BLyS. It was reported that the scFv version of the antibody binds human TR4 fusion protein with a binding affinity of Kd= 5.54 × 10^(-10) nM. It was also reported that when administered with in vivo Topotecan, this antibody was capable of retarding the growth of tumor cells in nude mice (US7064189).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
