Cat. No.
MABL-1444
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
12G9-2
From
Recombinant Antibody
Specificity
This antibody binds Dxd, an anti-cancer alkaloid camptothecin analog, which is a potent DNA topoisomerase I (TOP1) inhibitor. By forming a stable ternary complex with TOP1 and DNA, it arrests the cell cycle to S phase, thereby preventing DNA replication and causing cell death. DxD is used for the generation of antibody-drug conjugates for targeted delivery of toxic materials to cancerous cells. A HER2 targeting - DxD drug conjugate (DS-8201a) shows potent antitumor activity in HER2-positive models
Alternative Names
Exatecan derivative; Camptothecin analog
UniProt
Immunogen
The original antibody was generated by immunizing BALB/c mice with Dxd-KLH conjugate.
Application Notes
The binding reactivity of this antibody towards Dxd-KLH and Dxd-BSA conjugates was determined using ELISA. This antibody is capable of binding antibody-drug conjugates ADC-8201 and ADC029 in a competitive ELISA, wherein the antibody was trastuzumab. The binding affinity of this antibody towards Dxd-BSA was determined using surface plasmon resonance (SPR) and it was reported to be Kd= 3.38nM (CN115677857).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
