Cat. No.
MABL-1483
Application
IP, WB, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
7h2/1
From
Recombinant Antibody
Specificity
This antibody recognises human EME1. EME1 together with MUS81 create a DNA endonuclease which binds preferentially to branched DNA structures such as Holliday junctions and replication forks. It's engaged in processing of stalled or collapsed replication forks in mitosis.
Alternative Names
Essential meiotic endonuclease 1; Crossover junction endonuclease EME1; MMS4 homolog; hMMS4; EC 3.1.22; EC 3.1.22.; essential meiotic endonuclease 1 homolog 1 (S. pombe); essential meiotic endonuclease 1 homolog 2; FLJ31364; homolog of yeast EME1 endonuclease; MMS4L; MMS4; SLX2 structure-specific endonuclease subunit homolog A; SLX2A; MTA31
UniProt
Q96AY2
Immunogen
The antibody was raised against His-tagged recombinant Eme1 of human origin.
Application Notes
WB: MTA31 7h2/1 antibody was used to detect EME1 in western blot analysis in the experiments assessing EME1 as a potential marker of cisplatin resistance in cancer therapy (Tomoda et al., 2009). WB: This antibody was used to detect EME1 on western blots after previous immunopurification procedure (Laguette et al., 2014). IHC: Other group conducted an immunohistochemistry experiment staining against EME1 in formalin-fixed paraffin embedded esophageal adenocarcinoma tissue samples using MTA31 7h2/1 antibody (Dwalt et al., 2014).
Antibody First Published
Tomoda et al. Functional evidence for Eme1 as a marker of cisplatin resistance. Int J Cancer. 2009 Jun 15;124(12):2997-3001. doi: 10.1002/ijc.24268. PMID:19267403
Note on publication
This article describes the earliest found instance of application of MTA31 7h2/1 antibody which was used to detect EME1 in western blot analysis in the experiments assessing EME1 as a potential marker of cisplatin resistance in cancer therapy.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
