Cat. No.
MABL-1533
Application
IP, WB
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
CO1531
From
Recombinant Antibody
Specificity
This antibody is specific for human estrogen receptor. The estrogen receptor is a nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner (PubMed:20074560). Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability.
Alternative Names
Estrogen receptor beta; ESR2; ER-beta; Nuclear receptor subfamily 3 group A member 2
UniProt
Q92731
Immunogen
This antibody was raised by immunizing a rat with human estrogen protein.
Application Notes
The antibody was used for western blot on tissues of C57BL/6J wild-type mice (Montani et al, 2008; pmid:18281260). Whole-cell extracts from cells expressing ERα-LBD or full-length ERβ were immunoprecipitated with this antibody (Leong et al, 2005; pmid:16051668).
Antibody First Published
Leong et al. Recruitment of histone deacetylase 4 to the N-terminal region of estrogen receptor alpha Mol Endocrinol. 2005 Dec;19(12):2930-42. PMID:16051668
Note on publication
ERα N-terminal-specific coregulators were identified, a breast cDNA library was screened by T7 phage display and isolated histone deacetylase 4 (HDAC4).
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
