Cat. No.
MABL-1540
Application
dot blot assay, ICC, WB, IF
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
TP1170
From
Recombinant Antibody
Specificity
This antibody recognises mouse kappa light chain.
Alternative Names
antigen-binding fragment kappa light chain
UniProt
Immunogen
This antibody was raised by immunising alpacas with 1.0 mg polyclonal mouse IgG. Subsequently, the generation of nanobody immune libraries and the selection of antigen-specific nanobodies by phage display from these libraries were performed.
Application Notes
Anti-IgG nanobodies can often perform more efficiently than polyclonal secondary antibodies in various experimental assays. For instance, this nanobody when conjugated with the infrared fluorophore IRDye 800 was more efficient in terms of the detection sensitivity of IgG2a antibodies than the polyclonal secondary antibody via Western blot (Pleiner et al., 2018). TP1170 can also be used as an efficient secondary nanobody in the indirect immunofluorescence. It was used in combination with other nanobodies to detect both mouse IgG1 and IgG2a primary antibodies giving strong, clean signal without any background staining (Pleiner et al., 2018). This nanobody is also highly recommended for superresolution microscopy, as it can place the fluorophore much closer to the antigen which had been bound by the primary antibody than a usual secondary antibody would be able to (Pleiner et al., 2018).
Antibody First Published
Pleiner et al. A toolbox of anti–mouse and anti–rabbit IgG secondary nanobodies. J Cell Biol. 2018 Mar 5;217(3):1143-1154. doi: 10.1083/jcb.201709115. Epub 2017 Dec 20. PMID:29263082
Note on publication
The article describes the generation and characterisation of this nanobody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
