Cat. No.
MABL-1545
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Neisseria meningitidis
Clone No.
clone4
From
Recombinant Antibody
Specificity
This antibody binds a surface exposed epitope on N. meningitidis FHbp, a virulence factor critical for surviving of the bacteria in the human host. FHbp protein is a bacterial surface lipoprotein that binds host (human) complement factor H (fH, gene CFH). Neisseria meningitidis is the leading global cause of meningitis and sepsis.
Alternative Names
fHbp; GNA1870; Genome-derived Neisseria antigen 1870; Lipoprotein 2086
UniProt
E6MV22
Immunogen
The recombinant fHbp protein of Neisseria meningitidis was used as the antigen for the immunization of BALB/c mice. Anti-fHbp VH/k chain ribosome display libraries was generated and the actual antibody was obtained by panning the scFv library against the recombinant fHbp protein by using a single round of the ribosome display method via a rabbit reticulocyte lysate system.
Application Notes
This antibody exhibited high affinity and specificity in the enzyme-linked immunosorbent assay (ELISA) and the whole bacterial cell enzyme-linked immunosorbent assay (Bact- ELISA). The binding affinity of the scFv version of this antibody for fHbp was reported to be Kd= 7.03 nM (PMID: 29737965).
Antibody First Published
Yarian et al. Identification and characterization of a novel single-chain variable fragment (scFv) antibody against Neisseria meningitidis factor H-binding protein (fHbp) J Med Microbiol. 2018 Jun;67(6):820-827. PMID:29737965
Note on publication
Describes the isolation and characterization of an scFv antibody against fHbp protein of N meningitidis.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
