Cat. No.
MABL-1600
Application
immunoblot, in vivo, ELISA, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human, Mouse
Clone No.
NJB2
From
Recombinant Antibody
Specificity
This antibody is specific for the FN-EIIIB splice variant expressed in the ECM of tumors, metastases, and other diseases characterized by ECM deposition.
Alternative Names
FN-EIIIB; EDB domain of fibronectin
UniProt
Immunogen
The original antibody was isolated from an alpaca-derived phage display library, by panning against the EIIIB domain of fibronectin.
Application Notes
This nanobody was shown to detect primary tumors and metastatic sites in vivo by specifically binding to the ECM with excellent specificity in multiple models of breast cancer, including human and mouse triple-negative breast cancer, and in melanoma, as measured by PET/CT imaging with 64 Cu-NJB2. This nanobody recognized EIIIB-His by ELISA and in FN-containing EIIIB immunohistochemistry (IHC). Furthermore, its binding affinity to immobilized single EIIIB domain and a large recombinant rat FN fragment comprising FNIII7–15, including EIIIB (FN 7–15 EIIIB), was 1.9 × 10-9 M and 2.25 × 10-9 M, respectively, as determined by biolayer interferometry (BLI) (Jailkhani et al., 2019; PMID: 31068469). This nanobody was used for IHC of B16F10 and MC38 tumors to detect EIIIB expression (Xie et al., 2019; PMID: 30936321).
Antibody First Published
Jailkhani et al. Noninvasive imaging of tumor progression, metastasis, and fibrosis using a nanobody targeting the extracellular matrix Proc Natl Acad Sci U S A. 2019 Jul 9;116(28):14181-14190. doi: 10.1073/pnas.1817442116 PMID:31068469
Note on publication
The original publication presents the development and application of NJB2, a nanobody that targets disease-associated extracellular matrix, enabling noninvasive imaging of tumors, metastases, and fibrosis.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
