Cat. No.
MABL-1602
Application
inhibition, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Porphyromonas gingivalis
Clone No.
256-265-9
From
Recombinant Antibody
Specificity
The antibody reacts with conformational epitopes displayed by partially dissociated oligomers of FimA, but not with the 43 kDa FimA monomer. FimA is a structural protein of P. gingivalis fimbriae and is known to be involved in bacterial adherence to the host cell surface.
Alternative Names
Major fimbrium subunit FimA type-1; Fimbrillin; Fimbrilin; Major fimbrial subunit protein type I; fimbrial protein; fimbriae protein
UniProt
B2RH54
Immunogen
The original antibody was generated by immunizing BALB/c mice with FimA protein.
Application Notes
The antibody reacted with conformational epitopes expressed by partially dissociated oligomers, but not with monomer as elucidated by Western blot analysis. Ascites fluid containing the antibody showed the inhibitory activity against P. gingivalis to saliva-coated hydroxyapatite beads, an in vitro model for the pellicle-coated tooth surface. For example, 400-fold diluted monoclonal antibody inhibited the binding of the bacteria onto bead surfaces of 41.5%,(Koh et al., 2009; PMID: 20157608). The specificity of this antibody (IgG1) was confirmed by ELISA analysis. The original antibody detected FimA by western blot. The antibody significantly inhibited the binding of P. gingivalis to the surface of SHA beads by 51.81 ± 2.17%, when 50 ng/mL antibody was used (P 0.01). The antibody could be used as a basis for passive immunization against P. gingivalis-mediated periodontitis (Koh et al., 2011; PMID: 21223367).
Antibody First Published
Koh et al. Production of Monoclonal Antibodies Specific to FimA of Porphyromonas gingivalis and Their Inhibitory Activity on Bacterial Binding Immune Netw. 2009 Oct;9(5):203-7. PMID:20157608
Note on publication
The paper describes the generation and characterization of the antibody, which is specific to FimA. The antibody might be used as a basis for passive immunization against P. gingivalis- mediated periodontitis.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
