Cat. No.
MABL-1611
Application
Dot Blots, ELISA
Isotype
Engineer antibody
Species Reactivity
Ca. Liberibacter asiaticus
Clone No.
1199
From
Recombinant Antibody
Specificity
The antibody binds the flha protein of Ca. Liberibacterasiaticus.
Alternative Names
Flagellar biosynthesis protein FlhA
UniProt
C6XF33
Immunogen
The original antibody was generated by panning against a naturally expressed murine library.
Application Notes
Candidatus Liberibacter asiaticus (CaLas) is the pathogen that causes citrus Huanglongbing (HLB), the most serious disease of citrus. The paper describes the development of single chain variable fragment (scFv) antibodies against surface proteins of ‘Ca. Liberibacter asiaticus’ as potential diagnostic methods. A primary library of recombinant single chain variable fragment antibody was developed and the library was screened for antibodies that bind to specifically-chosen proteins that are present on the surface of CaLas. In particular, dot blot identified FlhA as a protein which is expressed on the surface of CaLas and ELISA result showed the antibody 1199 binds Flha (A450=0.26) (PMID 26744234).
Antibody First Published
Yuan et al. Development of single chain variable fragment (scFv) antibodies against surface proteins of 'Ca. Liberibacter asiaticus'. J Microbiol Methods. 2016 Mar;122:1-7. PMID:26744234
Note on publication
Development of a library of recombinant single chain variable fragment (scFv) antibodies which could be used as a diagnostic method for huanglongbing.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
