Cat. No.
MABL-1615
Application
SPR, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
EB10
From
Recombinant Antibody
Specificity
The antibody binds an epitope within domain D4 of human FLT3.
Alternative Names
FLK-2; FLT-3; STK-1; CD135; Fetal liver kinase 2; FL cytokine receptor; stem cell tyosine kinase 1; Fms-like tyrosine kinase 3; Receptor-type tyrosine-protein kinase FLT3
UniProt
P36888
Immunogen
The original Fab antibody was isolated from a human Fab phage display by selection of a high affinity antibody binding to the human FLT3.
Application Notes
The clone was shown to selectively bind to human FLT3 with high affinity, block FL binding and mediated potent anti-tumor activity in xenograft model by a mechanism involving activation of immune effector function. The ability of the antibody to bind the receptor was examined by ELISA (EC50 within the range 0.5-1 nM). The ability of the antibody to block receptor-ligand binding was examined in a FL binding competition ELISA, the antibody blocked the binding of FL to FLT3 with a IC50 within the range of 0.5-1 nM. The clone binds FLT3 with an affinity of 0.158 nM as determined by surface plasmon resonance (SPR). Flow cytometric analysis showed that the antibody bound to the wild-type FLT-3 expressed on EOL-1 cells and also to ITD-mutant FLT3 expressed on BaF3-ITD cells (US8071099B2).
Antibody First Published
Li et al. Anti-FLT3 antibodies
Note on publication
The patent describes a fully human antibody which specifically bind to human FLT3 within extracellular domains 4 or 5 with high affinity
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
