Cat. No.
MABL-1634
Application
crystallization, SPR
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
mAb4F5
From
Recombinant Antibody
Specificity
The antibody is specific for FVIIa. In particular, the epitope interacts with the periphery of the hydrophobic pocket.
Alternative Names
SPCA; Eptacog alfa; Proconvertin; Coagulation factor VII; EC：3.4.21.21; Blood coagulation factor VIIa; Serum prothrombin conversion accelerator
UniProt
P08709
Immunogen
The original antibody was generated by immunizing mice with FVIIaDVQ.
Application Notes
The binding affinity of original format of the antibody to FVIIaDVQ was measured by surface plasmon resonance (Kd= 32 pM). The crystal structure of the Fab fragment of the antibody was determined (Jiang et al., 2019; PMID： 31294329).
Antibody First Published
Jiang et al. Crystal structure, epitope, and functional impact of an antibody against a superactive FVIIa provide insights into allosteric mechanism Res Pract Thromb Haemost. 2019 Jul; 3(3)： 412–419. Published online 2019 Jun 20. doi： 10.1002/rth2.12211 PMID：31294329
Note on publication
The original paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
