Cat. No.
MABL-1651
Application
functional assay, FC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
UC7-13D5
From
Recombinant Antibody
Specificity
This antibody is specific for mouse gamma and delta TCR. TCR part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z.
Alternative Names
T-cell surface glycoprotein CD3 delta chain; T-cell receptor T3 delta chain; CD3d; T-cell surface glycoprotein CD3 gamma chain; T-cell receptor T3 gamma chain; CD3g
UniProt
P11942
Immunogen
This antibody was generated by immunizing a armenian hamster with murine murine gamma-delta TCR.
Application Notes
Alloreactive CD3'CD4- CD8- TCR y/6+ Tcell lines were used for a cytolytic assay. During the assay this antibody was used (Rellahan et al, 1991; pmid:1703211). Flow cytometry was preformed on the spleen cells of mice using this antibody (Koenecke et al, 2009; pmid:19130484). Flow cytometry was preformed on lymphoid cells of arthritic mice using this antibody (Peterman et al, 1993; pmid:8245484). A proliferation assay was preformed on inguinal lymph nodes from arthritic mice. The mice were treated with this antibody (Peterman et al, 1993; pmid:8245484).
Antibody First Published
Rellahan et al. Junctional Sequences Influence the Specificity of -y/b T Cell Receptors J Exp Med. 1991 Feb 1;173(2):503-6. PMID:1703211
Note on publication
To examine the role of V(D)J junctional sequences in antigen recognition by TCR- gamma/delta, an alloreactive murine TCR-gamma/delta+ T cell line, LKD1, specific for the I-Ad class II major histocompatibility complex (MHC) molecule, and compared its receptor with that expressed by a previously characterized class II MHC alloreactive T cell line, LBK5, specific for I-Ek,b,s Ia molecules was derived.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
