Cat. No.
MABL-1673
Application
in vitro, neutralization, SPR
Isotype
Engineer antibody
Species Reactivity
Poliovirus
Clone No.
PVSS21E
From
Recombinant Antibody
Specificity
This antibody is specific for Capsid proteins VP1 (GH loop, EF loop and C beta strand) and VP2 (EF loop) of the polio genome polyprotein.
Alternative Names
Capsid protein VP1; Capsid protein VP2; Poliovirus type 1
UniProt
P03300
Immunogen
The original antibody was raised by immunizing a dromedary with poliovirus type 1 Sabin strain.
Application Notes
The binding strength of the original antibody to poliovirus antigens was determined using surface plasmon resonance (Thys et al., 2011; PMID：21371506). The original antibody was used for a neutralization assay against three types of poliovirus. This antibody showed strong neutralization in type 1 polioviruses. The cytotoxicity of this antibody on HeLa cells was also evaluated. It showed no cytotoxicity (Thys et al., 2010; PMID：20566349). The effect of this antibody on virus entry and uncoating was determined. It showed change in the behavior of the polio particles (Schotte et al., 2014; PMID：24501405). The structure of this antibody was determined using cryo-EM (Strauss et al., 2016; PMID：26764003).
Antibody First Published
Thys et al. In vitro antiviral activity of single domain antibody fragments against poliovirus Antiviral Res. 2010 Aug;87(2)：257-64 PMID：20566349
Note on publication
The original paper aimed to determine whether VHHs inhibit wild-type and vaccine strains of poliovirus type 1.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
