Key features and details | |
Cat. No. | MABL-917 |
Name | Anti-CD39 mAbs |
Clone No. | AFD- mAb2 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | inhibition, ELISA, FC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for human CD39, also known as NTPDase 1 (ectonucleoside triphosphate diphosphohydrolase) or vascular CD39. CD39 works in concert with CD73 (ecto-5'- nucleotidase) to hydrolyze extracellular adenosine triphosphate (ATP) and adenosine diphosphate (ADP), generating adenosine. The resulting adenosine binds to adenosine receptors, inhibiting T-cell and natural killer (NK)-cell responses, thus suppressing immune system activity. Additionally, CD39 may play a role in preventing platelet aggregation by converting platelet-activating ADP into AMP. |
Alternative Name | ENTPD1; NTPDase 1; Ectonucleoside triphosphate diphosphohydrolase 1; Ecto-ATP diphosphohydrolase 1; Ecto-ATPDase 1; Ecto-ATPase 1; Ecto-apyrase; Lymphoid cell activation antigen |
UniProt | P49961 |
Immunogen | The original antibody was generated by humanizing an anti-CD39 murine antibody clone. |
Application Notes | The specificity of the original version of this antibody (Human IgG1, Fc Silent) was assessed using ELISA. Among the tested targets — vascular CD39 and the CD39 isoforms L1, L2, L3, and L4 — the antibody exclusively bound to vascular CD39. In a flow cytometry (FC) assay using Ramos human lymphoma cells, which express CD39, the antibody demonstrated strong binding. Additionally, the antibody exhibited potent inhibition of CD39 activity, as measured by a MALDI-TOF (Mass Spectrometry) assay, effectively blocking over 90% of CD39's enzymatic activity (US20220056145A1). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
