Cat. No.
MABL-1729
Application
immunocytochemistry, therapeutic, ELISA, FC, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
KM966
From
Recombinant Antibody
Specificity
This antibody recognizes and binds ganglioside GM2.
Alternative Names
GM2
UniProt
Immunogen
This chimeric antibody was generated by cloning the variable heavy and light chain sequences of murine parent antibody KM696 (IgM) and expressing it as a mouse/human IgG1. The parent antibody was raised against human GM2.
Application Notes
This antibody shows antitumor activity both in vitro and in vivo. Chimeric KM966 markedly suppressed the establishment of f human tumor xenografts in nude mice. Indirect immunofluorescence staining of tumor cell lines with the chimeric KM966 revealed that the antigen was expressed in substantial amounts on pulmonary tumor cells and leukemia cells as well as neuroectodermal origin tumor cells. This antibody also reacts with GM2 in an ELISA and also can be used for the identification of tumor cells expressing GM2 via flow cytometry (PMID: 8137257). The effect of chimeric antibody KM966 on GM2 expressing human solid tumors in vivo was done in PMID: 10417777. This antibody was also used in the immunocytochemical staining of cultured skin fibroblast of patients with GM2-gangliosidosis (PMID: 28417072). Humanized versions of this antibody KM8966, KM8967, KM8969 and KM8970 are available on request.
Antibody First Published
Nakamura et al. Chimeric anti-ganglioside GM2 antibody with antitumor activity. Cancer Res. (1994); 54(6):1511-6. PMID:8137257
Note on publication
Describes the generation of this chimeric antibody and its in vitro and in vivo characterization.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
