Cat. No.
MABL-1731
Application
IA, IAA, in vivo, IP, TLC, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
L612
From
Recombinant Antibody
Specificity
This antibody is specific for gangliosides GM3 and GM4, which contain a terminal NeuAc α2-3 Galactose residue.
Alternative Names
NeuAc α2-3 Galactose; NeuAc alpha 2-3 Galactose
UniProt
Immunogen
The original antibody was generated by transforming B-lymphocytes obtained from the lymph nodes of a breast cancer patient using the Epstein-Barr virus (EBV) transformation technique.
Application Notes
The original human IgM antibody’s reactivity was confirmed through various immunoassays, including immune adherence (IA) and immune adherence absorption (IAA) assays. It exhibited strong binding to gangliosides and effectively detected a range of human tumor tissues, such as melanoma, colon adenocarcinomas, and lung adenocarcinomas. The antibody demonstrated significant cytotoxic activity against antigen-positive tumor cells in the presence of complement. It showed notable reactivity with melanoma (62% positive) and lung cancer (100% positive) while showing less reactivity with other cancers. Neuraminidase treatment effectively inhibited its binding, affirming its specificity for gangliosides. The antibody has been used both therapeutically and diagnostically, showing efficacy in treating cutaneous malignant melanoma through intralesional injection, resulting in tumor necrosis and regression. It has been used to produce hybridomas that generate anti-idiotypic antibodies (anti-ids); beta- type anti-ids inhibit the antibody's binding and are useful for treatment and immunomodulation, while alpha-type anti-ids are used in diagnostic assays. Diagnostic applications include a three-step cell-ELISA and immunoperoxidase staining, both of which effectively detect GM3 antigens on tumor cells and tissue sections (US5419904A).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
