Cat. No.
MABL-1733
Application
Neutralisation, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
HIV
Clone No.
u5.5
From
Recombinant Antibody
Specificity
Specific to the principal neutralizing determinant (PND) of the V3 region of gp120 of HIV-1. It is reactive against peptides longer than 11 mer containing the sequence IHIGPGRAFYT (Okamoto et al, 1998).
Alternative Names
Ru5.5; gp120; Envelope glycoprotein gp160; Env polyprotein; transmembrane protein gp41; TM gp41; glycoprotein 41; gp41; surface protein gp120; SU gp120; mu5.5
UniProt
U5JCI1
Immunogen
Mice were immunised with HTLV-IIIMN cells. Spleen cells were harvested and hybridomas generated.
Application Notes
Has been tested against the primary field isolates of Japanese hemophiliacs seropositive for HIV-1 and protect SCID-hu mice from infection from these isolates. u5.5 was used as a humanized antibody and found to have a high neutralization activity. As of such it has been proposed as a protective antibody, however its epitope specificity gives it a limited range (Okamoto et al, 1998). It has been used in Western Blots to detect the V3 epitope of HIV-1 (Honda et al, 1995).
Antibody First Published
D’Souza, P Monoclonal antibodies to gp160: characterization of epitopes that elicit neutralizing activity. In The Third International Workshop Antibody Serological Project The Third International Workshop Antibody Serological Project. Keystone, National Institutes of Health, Bethesda, MD., p. 25.
Note on publication
There is no online record of this study, but it is referenced in the sequencing paper as the source of the antibody (Okamoto et al, 1998).
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
