Cat. No.
MABL-1740
Application
dot blot, inhibition assays
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
PDG7
From
Recombinant Antibody
Specificity
PDG7 does not bind to antigen denatured by heat of pH, indicating the necessity of intact GPIIb/IIIa.
Alternative Names
glycoprotein IIb/IIIa; integrin αIIbβ3;
UniProt
Immunogen
A phagemid library was constructed from the peripheral blood lymphocytes of a donor who was immunised with RhD+ red blood cells, and PDG7 was isolated after five rounds of affinity selection.
Application Notes
Dot blots confirmed the specificity of PDG7 for GPIIB/IIIA. Inhibition assays were used to show that platelet-associated autoantibodies from AITP patients binds a closely related epitope to that of the recombinant anti-GPIIb/IIIa phage clones (Escher et al, 1998). Inhibition studies also confirmed that PDG7 inhibited binding of eluted platelet-associated autoantibodies from AITP patients to platelets (Escher et al, 1998).
Antibody First Published
Escher et al, 1998. Recombinant human natural autoantibodies against GPIIb/IIIa inhibit binding of autoantibodies from patients with AITP Br J Haematol. 1998 Aug;102(3):820-8. PMID:9722313
Note on publication
Describes the production of the phage library and the characterisation of PDG7 through dot blots and inhibition assays.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
