Key features and details | |
Cat. No. | MABL-919 |
Name | Anti-CD3e mAbs |
Clone No. | AFD- 898H2-6-15 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | FC, IHC |
Species Reactivity | Pig |
Basic Information | |
Specificity | Hybridoma 898H2-6-15 was shown via double-colour flow cytometry analysis to bind swine lymphocyte populations specifically. It was further shown using FACS that purified 898H2-6-15 bound an epitope on CD3e shared by another anti-pig CD3 Ab (STH164). CD3, and the e chain in particular, is crucial for T cell receptor-dependent signal transduction across the lymphocyte membrane. |
Alternative Name | Leu4/T3; Cluster of Differentiation 3 epsilon chain; T cell glycoprotein CD3 epsilon chain |
UniProt | Q7YRN2 |
Immunogen | Murine antibodies were produced by immunising C3H/HeJ mice with swine peripheral blood lymphocytes. Hybridomas were generated by obtaining splenocytes from the immunised mice and fusing these with the non-Ig-producing myeloma cell line P3X63Ag8.653 via established methods. |
Application Notes | The initial identification of 898H2-6-15 showed that it recognised the T-cell receptor- associated glycoprotein CD3, in particular the e chain (Huang et al, 1999). More recently, the 898H2-6-15 VH and VL domains were reformatted into a scFv and conjugated to diphtheria toxin (Wang et al, 2011). This version of the antibody as an 'immunotoxin' has been shown to deplete circulating T cells in swine without observation of any clinical toxicities. |
Antibody First Published | Huang et al. Characterization of a monoclonal anti-porcine CD3 antibody Xenotransplantation 1999:5: 201-212 PMID:10503787 |
Note on publication | Describes the identification and qualitative characterisation of murine anti-pig CD3e antibodies from hybridoma. These were shown to be specific for pig T lymphocytes, as opposed to human and baboon peripheral blood cells, and mouse spleen cells. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
