Key features and details | |
Cat. No. | MABL-920 |
Name | Anti-CD3e mAbs |
Clone No. | AFD- PC3/188A |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, IHC |
Species Reactivity | Chicken, Cow, Guinea Pig, Horse, Pig, Primate, Rabbit, Rat, Human |
Basic Information | |
Specificity | This antibody recognises CD3 epsilon which is a part of the TCR-CD3 complex present on all mature T-lymphocyte cells and is crucial in the adaptive immunity. |
Alternative Name | Leu4/T3; T-cell surface glycoprotein CD3 epsilon chain; -cell surface antigen T3/Leu-4 epsilon chain; CD3e; CD3 epsilon; CD3e antigen; TCRE |
UniProt | P07766 |
Immunogen | This antibody was raised by immunising mice with a peptide sequence (156-168) from the intracytoplasmic portion of the epsilon-chain of CD3. |
Application Notes | This anti-CD3 has been used for the identification of normal and malignant T lymphocytes in paraffin embedded or frozen tissue sections. For instance, one group labelled paraffin- embedded pancreatic sections with PC3/188A antibody to identify T-lymphocytes while investigating the role of the oligodeoxynucleotide IMT504 on diabetes in rats (Bianchi et al., 2012). Another group also used this antibody to identify T lymphocytes by staining rat brain tissue samples against CD3 in a study investigating the mechanisms of neuroinflammation (Doursout et al., 2013). Finally, Roballo and Bushman (2019) utilised PC3/188A antibody as a marker of T cells during immunohistochemical analysis of frozen sciatic nerve sections. |
Antibody First Published | Jones et al. Detection of T and B cells in many animal species using cross- reactive anti-peptide antibodies. J Immunol. 1993 Jun 15;150(12):5429-35. PMID:8515069 |
Note on publication | This article describes generation and characterisation of PC3/188A antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
