Cat. No.
MABL-1759
Application
ICC, IP, WB, ELISA, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
BT164
From
Recombinant Antibody
Specificity
The epitope of BT164 was mapped in the N-terminal tail of histone H3 (27-KSAPAT-32) and included the apoptosis-induced trimethylation of K27.
Alternative Names
trimethyl-Histone H3 (Lys27); histone H3; H3K27; Trimethylated histone H3; Histone H3 trimethylated at Lysine 27; nucleosome; H3K27ME3; post translational modification; histone post-translational modification
UniProt
Immunogen
BT164 is a mice-derrived lupus antibody that was established by selection on apoptotic chromatin.
Application Notes
BT164 recognizes an apoptosis-induced trimethylation of K27 on N-terminal tail of histone H3 which is a target for autoantibodies in patients and mice with SLE (systemic lupus erythematosus) (van Bavel et al., 2011; pmid: 20699234). Consequently, this antibody is recommended for the detection and studying of SLE. ELISA studies demonstrated that BT164 exhibits preferential activity with the apoptotic nucleosomes in comparison with the control nucleosomes (van Bavel et al., 2011; pmid: 20699234). It can be used for the detection of apoptosis in general. Furthermore, this antibody was demonstrated to label cells with methylated histone-3 on K27 via immunofluorescence and it also immunoprecipitated chromatin with such a methylation profile, further demonstrating its ability to specifically recognize markers characteristic of SLE (van Bavel et al., 2011; pmid: 20699234).
Antibody First Published
van Bavel et al. Apoptosis-induced histone H3 methylation is targeted by autoantibodies in systemic lupus erythematosus. Ann Rheum Dis. 2011 Jan;70(1):201-7. doi: 10.1136/ard.2010.129320. Epub 2010 Aug 10. PMID:20699234
Note on publication
This article describes the generation and characterization of the antibody BT164.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
