Cat. No.
MABL-1773
Application
WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
2A1
From
Recombinant Antibody
Specificity
2A1 exhibited polyreactive recognition of peptide 100-119 from the alpha subunit and peptide 100-119 frmo the beta subunit. Moreover, 2A1 bound Hb and the Hb-haptoglobin complex with equal efficiency, implying different binding sites on Hb for haptoglobin and 2A1. 2A1 was also found to distinguish between Hb and metHb.
Alternative Names
Hemoglobin; Hb
UniProt
P01942
Immunogen
Murine hybridomas were generated using spleen cells from aging (8–10 month old) NZB/W F1 animals. Hybridomas secreting antibodies reactive to murine hemoglobin were subcloned by limiting dilution and antibody isotypes determined using commercial reagents. Eight-week-old NZB/W F1 were immunized subcutaneously with 50 mg mHb antigen emulsified in Complete Fruend’s Adjuvant.
Application Notes
2A1 has been used to bind to mHb in Western Blots and has been found to increase the secretion of TNF-alpha from endothelial cells when co-incubated with Hb, suggesting that 2A1 induces pro-inflammatory events (Bhatnagar 2011).
Antibody First Published
Bhatnagar et al. Serum and organ-associated anti-hemoglobin humoralautoreactivity: Association with anti-Sm responses and inflammation Eur. J. Immunol. 2011. 41: 537–548 PMID:21268022
Note on publication
Describes the presence of free hemoglobin in systemic lupus erythematosus and the investigation of anti-Hemoglobin autoimmune reactivity in patients.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
