Cat. No.
MABL-1780
Application
in vitro, in vivo, ELISA
Isotype
Engineer antibody
Species Reactivity
Rat, Human
Clone No.
FC44
From
Recombinant Antibody
Specificity
The antibody is specific for HCEC.
Alternative Names
Human cerebromicrovascular endothelial cells
UniProt
Immunogen
The original antibody was isolated from a naive phage displayed library of llama VHH. The library was panned against HCEC target cells.
Application Notes
The specificity of the original format of the antibody was confirmed by ELISA analysis (US8715659B2; Muruganandam et al., 2002; PMID: 11772942). The original format of the antibody bound to the vesicular and the membrane fractions of HCEC, and it was taken up by living HCEC, as shown by fluorescence microscopy. The antibody could transmigrate across an in vitro human blood-brain barrier (BBB) model. The antibody was also also detected in the brain after i.v. injection into mice (Muruganandam et al., 2002; PMID: 11772942). The antibody was able to transport across rat BBB, as demonstrated by in vitro and in vivo studies (Xiao et al., 2013; PMID: 23840214).
Antibody First Published
Muruganandam et al. Selection of phage-displayed llama single-domain antibodies that transmigrate across human blood-brain barrier endothelium FASEB J. 2002 Feb;16(2):240-2. doi: 10.1096/fj.01-0343fje. Epub 2001 Dec 28. PMID:11772942
Note on publication
The original paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
